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Lactobacillaceae are a large, diverse family of Gram-positive lactic acid-producing bacteria. As gut microbiota residents in many mammals, these bacteria are beneficial for health and frequently used as probiotics. Lactobacillaceae abundance in the gastrointestinal tract has been correlated with gastrointestinal pathologies and infection. Microbiota residents must compete for nutrients, including essential metal ions like calcium, zinc, and iron. Recent animal and human studies have revealed that dietary calcium can positively influence the diversity of the gut microbiota and abundance of intestinal Lactobacillaceae species, but the underlying molecular mechanisms remain poorly understood. Here, we investigated the impacts of calcium on the growth and biofilm formation of two distinct Lactobacillaceae species found in the gut microbiota, Lactobacillus acidophilus ATCC 4356 and Lactiplantibacillus plantarum ATCC 14917. We found that calcium ions differentially affect both growth and biofilm formation of these species. In general, calcium supplementation promotes the growth of both species, albeit with some variations in the extent to which different growth parameters were impacted. Calcium ions strongly induce biofilm formation of L. acidophilus ATCC 4356 but not L. plantarum ATCC 14917. Based on bioinformatic analyses and experimental chelator studies, we hypothesize that surface proteins specific to L. acidophilus ATCC 4356, like S-layer proteins, are responsible for Ca2+-induced biofilm formation. The ability of bacteria to form biofilms has been linked with their ability to colonize in the gut microbiota. This work shows how metal ions like Ca2+ may be important not just as nutrients for bacteria growth, but also for their ability to facilitate cell-cell interactions and possibly colonization in the gut microbiota. 

ABSTRACT AimTo quantify the intra‐community variability of leaf‐out (ICVLo) among dominant trees in temperate deciduous forests, assess its links with specific and phylogenetic diversity, identify its environmental drivers and deduce its ecological consequences with regard to radiation received and exposure to late frost. LocationEastern North America (ENA) and Europe (EUR). Time Period2009–2022. Major Taxa StudiedTemperate deciduous forest trees. MethodsWe developed an approach to quantify ICVLo through the analysis of RGB images taken from phenological cameras. We related ICVLo to species richness, phylogenetic diversity and environmental conditions. We quantified the intra‐community variability of the amount of radiation received and of exposure to late frost. ResultsLeaf‐out occurred over a longer time interval in ENA than in EUR. The sensitivity of leaf‐out to temperature was identical in both regions (−3.4 days per °C). The distributions of ICVLo were similar in EUR and ENA forests, despite the latter being more species‐rich and phylogenetically diverse. In both regions, cooler conditions and an earlier occurrence of leaf‐out resulted in higher ICVLo. ICVLo resulted in ca. 8% difference of radiation received from leaf‐out to September among individual trees. Forest communities in ENA had shorter safety margins as regards the exposure to late frosts, and were actually more frequently exposed to late frosts. Main ConclusionsWe conducted the first intercontinental analysis of the variability of leaf‐out at the scale of tree communities. North American and European forests showed similar ICVLo, in spite of their differences in terms of species richness and phylogenetic diversity, highlighting the relevance of environmental controls on ICVLo. We quantified two ecological implications of ICVLo (difference in terms of radiation received and exposure to late frost), which should be explored in the context of ongoing climate change, which affects trees differently according to their phenological niche. 

Environmental observation networks, such as AmeriFlux, are foundational for monitoring ecosystem response to climate change, management practices, and natural disturbances; however, their effectiveness depends on their representativeness for the regions or continents. We proposed an empirical, time series approach to quantify the similarity of ecosystem fluxes across AmeriFlux sites. We extracted the diel and seasonal characteristics (i.e., amplitudes, phases) from carbon dioxide, water vapor, energy, and momentum fluxes, which reflect the effects of climate, plant phenology, and ecophysiology on the observations, and explored the potential aggregations of AmeriFlux sites through hierarchical clustering. While net radiation and temperature showed latitudinal clustering as expected, flux variables revealed a more uneven clustering with many small (number of sites < 5), unique groups and a few large (> 100) to intermediate (15–70) groups, highlighting the significant ecological regulations of ecosystem fluxes. Many identified unique groups were from under-sampled ecoregions and biome types of the International Geosphere-Biosphere Programme (IGBP), with distinct flux dynamics compared to the rest of the network. At the finer spatial scale, local topography, disturbance, management, edaphic, and hydrological regimes further enlarge the difference in flux dynamics within the groups. Nonetheless, our clustering approach is a data-driven method to interpret the AmeriFlux network, informing future cross-site syntheses, upscaling, and model-data benchmarking research. Finally, we highlighted the unique and underrepresented sites in the AmeriFlux network, which were found mainly in Hawaii and Latin America, mountains, and at under- sampled IGBP types (e.g., urban, open water), motivating the incorporation of new/unregistered sites from these groups. 

Abstract Two reports of Antarctic Region potential new record high temperature observations (18.3°C, 6 February 2020 at Esperanza station and 20.8°C, 9 February 2020 at a Brazilian automated permafrost monitoring station on Seymour Island) were evaluated by a World Meteorological Organization (WMO) panel of atmospheric scientists. The latter figure was reported as 20.75°C in the media. The panel considered the synoptic situation and instrumental setups. It determined that a large high-pressure system over the area created föhn conditions and resulted in local warming for both situations. Examination of the data and metadata of the Esperanza station observation revealed no major concerns. However, analysis of data and metadata of the Seymour Island permafrost monitoring station indicated that an improvised radiation shield led to a demonstrable thermal bias error for the temperature sensor. Consequently, the WMO has accepted the 18.3° C value for 12 noon (LST) 6 February 2020 [1500 UTC 6 February 2020] at the Argentine Esperanza station as the new “Antarctic Region [continental, including mainland and surrounding islands] highest temperature recorded observation” but rejected the 20.8° C observation at the Brazilian automated Seymour Island permafrost monitoring station as biased. The committee strongly emphasizes the permafrost monitoring station was not badly designed for its purpose, but the project investigators were forced to improvise a non-optimal radiation shield after losing the original covering. Secondly, with regard to media dissemination of this type of information, the committee urges increased caution in early announcements as many media outlets often tend to sensationalize and mischaracterize potential records. 

Abstract The influence of aquaporin (AQP) activity on plant water movement remains unclear, especially in plants subject to unfavorable conditions. We applied a multitiered approach at a range of plant scales to (i) characterize the resistances controlling water transport under drought, flooding, and flooding plus salinity conditions; (ii) quantify the respective effects of AQP activity and xylem structure on root (Kroot), stem (Kstem), and leaf (Kleaf) conductances; and (iii) evaluate the impact of AQP-regulated transport capacity on gas exchange. We found that drought, flooding, and flooding plus salinity reduced Kroot and root AQP activity in Pinus taeda, whereas Kroot of the flood-tolerant Taxodium distichum did not decline under flooding. The extent of the AQP control of transport efficiency varied among organs and species, ranging from 35–55% in Kroot to 10–30% in Kstem and Kleaf. In response to treatments, AQP-mediated inhibition of Kroot rather than changes in xylem acclimation controlled the fluctuations in Kroot. The reduction in stomatal conductance and its sensitivity to vapor pressure deficit were direct responses to decreased whole-plant conductance triggered by lower Kroot and larger resistance belowground. Our results provide new mechanistic and functional insights on plant hydraulics that are essential to quantifying the influences of future stress on ecosystem function.